The Quantitative Determination of Thyroxine Binding Globulin by Laurell Electrophoresis
نویسندگان
چکیده
منابع مشابه
Assay of thyroxine-binding globulin by polyacrylamide electrophoresis: normal values.
Thyroxine-binding globulin (TBG) assays by the polyacrylamide electrophoresis assay of Nikolai and Seal have been carried out on 315 normal subjects. The assay was carried out with 200 exogenous labeled thyroxine per 100 ml added to the samples. The means and standard deviations for the three thyroxine-binding proteins in males (n = 140) were: TBG 19.7 ±3.0 p.g/100 ml, thyroxine-binding albumin...
متن کاملThyroxine-Binding Globulin Concentrations
Plasma thyroid hormone concentrations may be abnormal in non-thyroid disease and pose diagnostic problems. For example, concentrations of triiodothyronine (T3) in the blood decline in acute illness with a reciprocal increase in reverse triiodothyronine (reverse T3), but thyroxine (T4) concentrations are usually normal or only slightly decreased (1, 2). However, McLarty et al. (3) reported that ...
متن کاملFamilial thyroxine-binding globulin deficiency.
Genetically determined thyroxine-binding globulin deficiency is described in two families in the United Kingdom. All subjects in both pedigrees were euthyroid. Transmission was by sex-linkage; males showed low serum protein bound iodine and high thyroxine (T4) resin uptake due to complete absence of serum thryroxine-binding globulin; females were less severely affected. The distinctive biochemi...
متن کاملCharacterization of the thyroxine-binding site of thyroxine-binding globulin by site-directed mutagenesis.
The principal transport protein for T4 in human blood, thyroxine-binding globulin (TBG), binds T4 with an exceptionally high affinity (Ka = 10(10) M(-1)). Its homology to the superfamily of the serpins has recently been used in the design of chimeric proteins, providing experimental evidence that an eight-stranded beta-barrel domain encompasses the ligand-binding site. We have now characterized...
متن کاملDetermination of thyroxine-binding globulin in human serum by single radial immunodiffusion and radioimmunoassay.
Two immunochemical methods for determination of thyroxine-binding globulin in human serum were developed, in which the purified globulin and monospecific antiserum to it are used. One method, based on radial immunodiffusion, has good precision and values for analytical recovery. Reference values obtained for men were 9.8-17.8 mg/liter and for women 11.3-20.5 mg/liter. The sex-related difference...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1974
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1974.12.3.124